《植物生理学报》 2013, 49(5): 477-484

叶绿体分裂突变体cpd4中突变基因ARC5的鉴定与分析

沈鑫曌¹, 刘含¹, 刘晓庆¹, 袁光孝¹, 高岳芳^1,*, 高宏波^1,2,*

北京林业大学1生物科学与技术学院, 2林木育种国家工程实验室, 北京100083

通信作者：高岳芳；E-mail: gaobjfu@yahoo.com, yuefanggao@126.com；Tel: 010-62336496

摘　要：

cpd4是一个以Col为背景的拟南芥叶绿体分裂突变体, 其突变表型与以Ler为背景的突变体arc5-1表型相似, 即叶绿体数量明显减少, 体积明显增大且形状常呈哑铃型。该突变未能对叶绿素含量造成明显影响。遗传分析显示其突变表型受隐性单基因控制。通过图位克隆的方法确定其突变性状是由ARC5基因突变引起的。该突变影响了ARC5基因mRNA的正常剪切, 对mRNA的稳定性也造成严重影响。该工作为进一步研究ARC5在叶绿体分裂中的作用提供了有用的材料和信息。

关键词：拟南芥; 叶绿体分裂; ARC5

收稿：2013-03-21   修定：2013-04-08

资助：资助 国家自然科学基金(30971439和J1103516)和北京市自然科学基金(5102022)。 * 共同通讯作者

Identification and Analysis of the Mutant Gene ARC5 in a Chloroplast Division Mutant cpd4

SHEN Xin-Zhao¹, LIU Han¹, LIU Xiao-Qing¹, YUAN Guang-Xiao¹, GAO Yue-Fang^1,*, GAO Hong-Bo^1,2,*

¹College of Biological Sciences and Biotechnology, ²National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China

Corresponding author: GAO Yue-Fang; E-mail: gaobjfu@yahoo.com, yuefanggao@126.com; Tel: 010-62336496

Abstract:

cpd4 (chloroplast division 4) is a chloroplast division mutant isolated from Columbia background. It has a phenotype similar to that of arc5-1, a mutant from Landsberg erecta background, i.e., fewer and larger dumbbell shaped chloroplasts. The chlorophyll content was not obviously affected by the mutation in cpd4. Ge-netic analysis indicated that the mutant phenotype was controlled by a single recessive gene. The gene respon-sible for the mutant phenotype was identified to be ARC5 through a map-based cloning strategy. The mutation seriously affected the correct splicing and the stability of ARC5 mRNA. This work provides some useful mate-rials and information for further studying the function of ARC5.

Key words: Arabidopsis thaliana; chloroplast division; ARC5